Effect of Kapalbhati on Blood Pressure in Naive

Background and Objectives: Kapalbhati is among one of the cleansing act (shatkarma) in yogic philosophy. It is highly recommended for those who have to do great deal of study and need a clean, clear mind. This study was conducted among medical students at Nepalgunj medical college, Nepalgunj, Nepal.Material and Methods: This analytical study was conducted among 100 medical students of Nepalgunj Medical College, Nepalgunj, Nepal who gave consent and performed Kapalbhati correctly were included in the study. SBP and DBP were the cardiac parameters taken. Those parameters were taken before, immediately, after 1 minute, 2 minutes and 3 minutes of Pranayama. The data were entered in SPSS and Statistical analysis was done using version 23.Results: As in usual exercises, SBP and DBP increases significantly immediately after Kapalbhati session when compared with the value before exercises. The basal (pre-Kapalbhati) mean SBP and DBP were 125.76 ± 7.36 and 82.92 ± 5.75 which increases up to 143.60 ± 11.18 and 90.33 ± 7.90 respectively immediately after exercises and fall after 3 minutes of exercises value being 127.05 ± 10.93 and 81.38 ± 5.38 respectively.Conclusion: There is significant rise between Pre-value and post-value (immediate) of all parameters and significant fall of all parameters if compared between post-value and after 3 minute.

Batch production and media optimization of anti-leukemic L-asparaginase from Pseudomonas fluorescens by Taguchi DOE methodology.

L-Asparaginase isolated from bacterial sources has been shown to possess antileukemic characteristic, mainly against the acute lymphoblastic leukemia. Large number of bacterial strains have been reported which can produce it. The production level of the enzyme by the bacterial sources is very low and hence the factors have to be distinguished which effect the growth of cells and production of enzyme directly or indirectly, in order to decrease the cost of treatment. The production of L-Asparaginase was optimized in classical fermentation process by the use of Taguchi DOE methodology. L-18 array was selected for the purpose of media optimization. The five factors at three levels were considered for the optimization. L-Asparaginase production was significantly (p<0.05) affected by the interaction of two factors present in the culture broth. The factors interaction viz. energy source-nitrogen source, energy source-phosphate source etc. having pronounced effect on the production while individually they have the minimum impact on L-Asparaginase production. So the interactions of different parameters were studied and they were used to formulate the optimized condition for the production of LAsparaginase. After the validation of result by performing the experiment under optimized condition there was about 28.48% increase in the production of the enzyme.